Separation and Purification of Coenzyme Q10 from Rhodobacter sphaeroides

碩士 === 元智大學 === 化學工程與材料科學學系 === 95 === Coenzyme Q10 acts as an essential component of adenosine triphosphate (ATP) generation in the oxidative phosphorylation chain and as an antioxidant preventing lipid peroxidation and scavenging superoxide. The purpose of this study will focus on separation from...

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Main Authors: Jiun-Jie Tsai, 蔡俊杰
Other Authors: Ho-Shing Wu
Format: Others
Language:en_US
Published: 2007
Online Access:http://ndltd.ncl.edu.tw/handle/60514795386679781900
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spelling ndltd-TW-095YZU051590422016-05-23T04:17:54Z http://ndltd.ncl.edu.tw/handle/60514795386679781900 Separation and Purification of Coenzyme Q10 from Rhodobacter sphaeroides 自Rhodobactersphaeroides分離與純化輔酵素-Q10之研究 Jiun-Jie Tsai 蔡俊杰 碩士 元智大學 化學工程與材料科學學系 95 Coenzyme Q10 acts as an essential component of adenosine triphosphate (ATP) generation in the oxidative phosphorylation chain and as an antioxidant preventing lipid peroxidation and scavenging superoxide. The purpose of this study will focus on separation from the fermentation broth and purification of CoQ10 that produced by Rhodobacter sphaeroides BCRC 13100. Three topics were discussed as follows:(1) Disruption methods:the cells were disrupted by using enzyme, freezing and heating, chemicals or high press homogenizer. The results showed that the product yield could achieve 2.85 mg/g-DCW for enzyme method but its cost was higher. Secondly, the method of high press homogenizer was better that the product yield can achieve 2.11 mg/g-DCW. On the other hand, the ethanol could be used to disrupt and extract directly. The product yield could also achieve 2.01 mg/g-DCW. (2) Separation methods:CoQ10 was quantitatively extracted with various organic solvents. Using ethanol twice extraction could separate the products to release into the surrounding solution.CoQ10 was extracted by ethanol at 40℃ for 30min. The product yield could also achieve 2.46 mg/g-DCW. The product yield of ethanol disrupted method is 0.86 fold of the product yield using enzyme method. (3) Purification method:The crude extract was purified by silica gel column chromatography. Another method was liquid-liquid extraction with hexane. After crystallization, the purity determined by means HPLC could achieve 96.9%. Ho-Shing Wu 吳和生 2007 學位論文 ; thesis 79 en_US
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language en_US
format Others
sources NDLTD
description 碩士 === 元智大學 === 化學工程與材料科學學系 === 95 === Coenzyme Q10 acts as an essential component of adenosine triphosphate (ATP) generation in the oxidative phosphorylation chain and as an antioxidant preventing lipid peroxidation and scavenging superoxide. The purpose of this study will focus on separation from the fermentation broth and purification of CoQ10 that produced by Rhodobacter sphaeroides BCRC 13100. Three topics were discussed as follows:(1) Disruption methods:the cells were disrupted by using enzyme, freezing and heating, chemicals or high press homogenizer. The results showed that the product yield could achieve 2.85 mg/g-DCW for enzyme method but its cost was higher. Secondly, the method of high press homogenizer was better that the product yield can achieve 2.11 mg/g-DCW. On the other hand, the ethanol could be used to disrupt and extract directly. The product yield could also achieve 2.01 mg/g-DCW. (2) Separation methods:CoQ10 was quantitatively extracted with various organic solvents. Using ethanol twice extraction could separate the products to release into the surrounding solution.CoQ10 was extracted by ethanol at 40℃ for 30min. The product yield could also achieve 2.46 mg/g-DCW. The product yield of ethanol disrupted method is 0.86 fold of the product yield using enzyme method. (3) Purification method:The crude extract was purified by silica gel column chromatography. Another method was liquid-liquid extraction with hexane. After crystallization, the purity determined by means HPLC could achieve 96.9%.
author2 Ho-Shing Wu
author_facet Ho-Shing Wu
Jiun-Jie Tsai
蔡俊杰
author Jiun-Jie Tsai
蔡俊杰
spellingShingle Jiun-Jie Tsai
蔡俊杰
Separation and Purification of Coenzyme Q10 from Rhodobacter sphaeroides
author_sort Jiun-Jie Tsai
title Separation and Purification of Coenzyme Q10 from Rhodobacter sphaeroides
title_short Separation and Purification of Coenzyme Q10 from Rhodobacter sphaeroides
title_full Separation and Purification of Coenzyme Q10 from Rhodobacter sphaeroides
title_fullStr Separation and Purification of Coenzyme Q10 from Rhodobacter sphaeroides
title_full_unstemmed Separation and Purification of Coenzyme Q10 from Rhodobacter sphaeroides
title_sort separation and purification of coenzyme q10 from rhodobacter sphaeroides
publishDate 2007
url http://ndltd.ncl.edu.tw/handle/60514795386679781900
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AT càijùnjié zìrhodobactersphaeroidesfēnlíyǔchúnhuàfǔjiàosùq10zhīyánjiū
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