Study on the anti-cancer and anti-oxidation properties of Tithonia diversifolia.

• Main Authors: Chan Chung-Hsien, 詹忠憲
• Other Authors: Lo Jiunn-Guang
• Format: Others
• Language: zh-TW
• Published: 2007
• Online Access: http://ndltd.ncl.edu.tw/handle/9hjp95

碩士 === 元培科學技術學院 === 影像醫學研究所 === 95 === Abstract The liver cancer is one of the ten greatest deaths in Taiwan. Tithonia diversifolia, the Composite plant, is the folk medicine to cure the liver cancer, sarcoma and skin cancer in Taiwan. In this study, two kinds of extracts (extracted by high-pressure water and methanol extraction) were tested the properties anti-cancer property in prostate cancer cells (DU-145) and hepatoma cells (Huh-7) and anti-oxidation properties in Mouse's normal liver cells (clone-9). The preliminary results show that the methanol extracts have anti-cancer effects in DU-145 and Huh-7 cells. After 24, 48 and 72 hours treatments with the extracts for DU-145, the cells’ growths were dramatically inhibited, and the IC50 was evaluated about 2.5μg/ml. For Huh-7 cells, the IC50 was evaluated about 4.5μg/ml after 24 hours treatment and 3μg/ml after 48 and 72 hours treatments. In the other way, the preliminary result shows that the high-pressure water extracts don’t have the anti-cancer effects. In the anti-oxidant experiments, the cells were treated with the exteacts of Tithonia diversifolia to prevent the H2O2-induced damage in Mouse’s normal liver cells (clone-9). The results show that high-pressure water extracts can protect cells to resistant H2O2-induced oxidation damage.