| Summary: | 碩士 === 國立陽明大學 === 微生物及免疫學研究所 === 95 === Transforming growth factor-beta1 (TGF-β1) is one of the major secreted cytokines in patients with acute or chronic HBV infection. Additionally, TGF-β1 plays an important role in regulation of cellular functions and immune responses. In first part of this study, we expressed TGF-β1 via recombinant adenovirus in HBV-producing stable cell lines to study effects of TGF-β1 on HBV replication in vitro. The results demonstrated that the effects of TGF-β1 could reduce cytoplasmic HBV nucleocapsids, and secreted HBV particles in the actively dividing HBV-producing cell lines. In the second part of this study, we further expressed TGF-β1 via recombinant adenovirus in HBV-transgenic mice to study the effect of TGF-β on HBV replication in vivo. We demonstrated that TGF-β1 could reduce cytoplasmic nucleocapsids in livers of HBV-transgenic mice in a dose dependent manner. However, TGF-β1 could not reduce HBV particles in the plasma of HBV-transgenic mice.
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