Determination of small biomolecule by fiber-optics surface plasma resonance sensor

• Main Authors: Chien Pan, 潘杰
• Other Authors: Ming-Tse Lin
• Format: Others
• Language: zh-TW
• Published: 2007
• Online Access: http://ndltd.ncl.edu.tw/handle/7a9z8r

碩士 === 大同大學 === 生物工程學系(所) === 95 === Fiber-optic surface plasmon resonance (SPR) can be used as a rapid and quantitative assay, and is also an important tool for monitoring biomolecular interactions. Furthermore, the use of fiber-optic SPR provides a number of potential advantages. Because the fiber optic sensors are reproducible and inexpensive to be disposable, binding kinetics and screening analyses can be performed in complex media without fear of irreversibly fouling expensive chipbased SPR platforms. Additionally, the small size of the fibers optic probe and sensing area make the sensors ideal for studying small volumes of samples. The applications are more and more than before. In this study, we used the fiber-optic SPR sensor designed and obtained from Graduate Institute of Electro-Optical Engineering in Tatung University to determine the small biomolecules. The nanogold particle and precipitation of 4-choro-1-naphthol were used to amplify the detection signals of SPR. The amplification method using nanogold particle was insignificant, but one using precipitation by reducing 4-choro-1-naphthol was able to enhance significantly the SPR signals. The signal changes of wavelength shift were consistent to the increasing in the concentrations of biotin-conjugated albumin bovine serum (biotin-BSA) and ochratoxin A-conjugated ovalbumin (OTA-OVA). Therefore, these results indicated that the amplification method of reducing precipitation by enzyme was suitable to the fiber-optic SPR detection system. The detection limiting for biotin-BSA was 0~10 �慊/ml, and 10~20 ng/ml for biotin.