Fabrication and properties of PDMS-based flow-through PCR device

• Main Authors: Wei-guo Gu, 古偉國
• Other Authors: Chi-yang Yu
• Format: Others
• Language: zh-TW
• Published: 2007
• Online Access: http://ndltd.ncl.edu.tw/handle/5zzz4a

碩士 === 大同大學 === 生物工程學系(所) === 95 === Polymerase chain reaction (PCR) utilizes DNA polymerase to amplify specific DNA fragment. The reaction is carried out under three different reaction temperatures, and the amplified DNA product can be used in subsequent analysis and research. In this thesis, a PDMS-based flow-though PCR device was fabricated using micro-fabrication techniques. The flow channel (50 μm deep) plate and a 1 mm-thick flat back plate were fabricated using PDMS, then the two plates were bonded after oxygen plasma treatment. A thin-film chromium resistive heater was manufactured on Corning 1737 glass substrate. The PCR device was assembled simply by placing the PDMS flow channel on the top of the glass heating chip. The cDNA of D-amino acid oxidase (from Trigonopsis variabilis) was used as the DNA template. The effects of flow rate and cross contamination on the efficiency of the PCR device were studied. In addition, the effects of surface modification by BSA, Pluronic® F-68, Pluronic® F-127, and neat silane were discussed.