Part 1：The Effect of Carbopol on ALA-induced PDT in CL1 cellsPart 2：Establish the Skeleton of Safety Evaluation and Standard Regulation in Chemical Sunscreen Products

• Main Authors: Wei-Ling Li, 李維靈
• Other Authors: Tsui-Min Tsai
• Format: Others
• Language: zh-TW
• Published: 2007
• Online Access: http://ndltd.ncl.edu.tw/handle/76931563372087271394

碩士 === 臺北醫學大學 === 生物醫學材料研究所 === 95 === Part 1： Photodynamic therapy (PDT) is a novel methodology used in the treatment of neoplastic lesion. Due to its safety, efficiency, and minimal invasion, PDT has become more popular in the treatment of gastrointestinal cancers, esophageal cancer, and lung cancer. Aminolevulinic acid (ALA) was applied in this study. PDT employing ALA as a precursor of the photosensitizer Protoporphyrin IX (PpIX) has become a promising approach to treat superficial cancers. ALA has been successfully used to diagnose and treat neoplastic tissue. In this study, we tried to investigate the potential of using ALA-PDT to treat the lung adenocarcinoma, and the role of the excipient Carbopol 971P (CP971P) on the formation of PpIX. Human lung adenocarcinoma cell lines, CL1-0 and CL1-2 cell lines (obtained from a 64-year-old male in Taiwan) with various in vitro invasive abilities were used in this study. Compared to CL1-2 cells, PpIX formation was more in the CL1-0 cells. Significant phototoxicity was observed in CL1-0 while CL1-2 cells were less sensitive to ALA-PDT. These results indicate that, higher invasive ability of CL1-2 cells express less sensitive to ALA-PDT induced phototoxicity. The presence of CP971P during the ALA incubation seemed to enhance the phototoxicity in CL1-0 cells but not in CL1-2 cells. Part 2： The sunscreen products had already become the people''s livelihood necessities in recent years. However, the safety of chemical sunscreen ingredients had been questioned and world-wide discussed for the past. Large numbers of countries have already invested lots of finance in sunscreen products. Global cosmetic harmonization of the regulations that control these products is a priority for all over the world. The competition of marketing is fierce in the whole world. In order to get the best existence space, it is already imperative to make and manage to cosmetic products. In this study, we aimed to compile the safety and assessment methods to inspect the chemical sunscreens products. We tried to establish the data base of the interactions between sunscreen ingredients and other cosmetic compositions. We searched the regulation rules of sunscreens in U.S., European Union, Australia and Japan, and compared the management and inspection of the chemical sunscreens. We proposed a regulation scheme of chemical sunscreens to fit the folkway in Taiwan under global harmonization.