Molecular Fingerprinting Database Establishment of Acinetobacter baumannii in Taiwan and the Study of Antibiotic Resistance of Integron.

• Main Authors: Ya-Ting Tsao, 操雅婷
• Other Authors: 吳瑞裕
• Format: Others
• Language: zh-TW
• Published: 2007
• Online Access: http://ndltd.ncl.edu.tw/handle/65038658099611452756

碩士 === 臺北醫學大學 === 醫學研究所 === 95 === Because of the misuse in antibiotics administration, bacteria with drug resistance have been increased and transformed dramatically. Acinetobacter baumannii has become one of the most important clinical pathogens in these years. Recently multidrug-resistant A. baumannii (MDR-AB) has high occurrence involving in nosocomial outbreak in several Taiwan medical centers, and also been demonstrated to against the third generation β-lactam, such as imipenem. Therefore, A. baumannii now is recognized as one of the most difficult grem-negative bacteria to deal with in clinical treatment and epidemiology. Rapid and accurate methods to identify the bacterial species and genotype are the most critical issue in clinical treatment and epidemiological study. ERIC (Enterobacterial repetitive intergenic consensus sequence) and REP (repetitive extragenic palidromic sequence) PCR have demonstrated with excellent discriminative capability among bacterial pathogens, and were used for genotyping of A. baumannii to establish full database in our previous research. Many studies have shown that integron play an important role involving in the horizontal transfer of antibiotic resistance genes among different bacterial species. Gene cassette could carry large varieties of resistance genes within integron through site-specific recombination to move-in or move-out the integron. We first obtained full length of gene cassette through integron PCR, then analyzed each resistance gene using DNA sequencing and NCBI sequence blast. Extended-spectrum β-lactamase is the enzyme defined as the ability hydrolyze to the second-or third-generation of β-lactam antibiotics. Several common ESBL types are identified with highly occurring in variety of AB or MDR-AB strains such as TEM and SHV, this study can establish a standard platform for accurate bacterial identification system that may provides a novel treatment study to bacterial infection in clinical.