| Summary: | 碩士 === 臺北醫學大學 === 細胞及分子生物研究所 === 95 === The matrix of human umbilical cord is what we called Wharton’s jelly (WJ). The cells in Wharton’s jelly were successfully isolated from human umbilical cord and cultured several passages in vitro. Wharton’s jelly has characteristic of mesenchymal stem cells and could differentiate to adipocyte, chondrocyte, osteocyte and glia cells.
Mesenchymal stem cells were found on human arterial wall in recently studies. It has the potential to differentiate to adipocyte and osteocyte.
We isolated Human Umbilical Artery Cells (hUAMPs) from normal human umbilical arteries. To know the characteristic of hUAMPs, we cultured this cells with two kinds of condition medium (DMEM + 20% FBS and EGM-2 + 10% FBS). They were successfully maintained several passages in vitro and have good proliferation rate. The morphology of hUMAPs is fibroblast-like and their doubling times are 23hr and 25hr. Using the flow cytometric analysis, we found that mesenchymal stem cell surface markers (CD29、CD44、CD49d、CD49e、CD49f、CD105、CD166、CDw90、SH2、SH4、HLA-ABC) are positive on hUAMPs cultured in both DMEM + 20% FBS and EGM-2 + 10% FBS. But CD14 and CD31 could only be detected on hUAMPs surface which cultured in EGM-2 + 10% FBS.
We also found that hUAMPs cultured in both medium has the ability to differentiate into chondrocyte, osteocyte, adipocyte, neurocyte. Capillary can be observed on tube formation assay only in hUAMPs cultured with EGM-2 + 10%FBS.
We concluded all the results and defined that hUAMPs may be one of mesenchymal stem cells which play an important role on future medical treatment.
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