The effect of static magnetic field on lipopolysaccharide induced excessive immune reaction – in vivo investigations on BALB/cByJNarl mice

• Main Authors: Chih-Ping Lin, 林致平
• Other Authors: Che-Tong Lin
• Format: Others
• Language: zh-TW
• Published: 2007
• Online Access: http://ndltd.ncl.edu.tw/handle/14534685114767318449

碩士 === 臺北醫學大學 === 牙醫學系碩博士班 === 95 === Clinical observations found that patients suffered from bacterial-induced inflammation which result in serious periodontal disease have gradually increased over the years. Lipopolysaccharide (LPS), found in the cell wall of bacteria, was the toxic substance responsible for the excessive immune reaction such as sepsis and septic shock, as well as the periodontal disease. However, drugs developed based on the above mechanism showed no significant clinical breakthrough. Therefore, it seems to be a bias to find a non-invasive and physical treatment in clinical research. This study aims to investigate the probability of increasing the survival rate of static magnetic field (SMF)-exposed mice challenged with LPS. In this study, LPS was intravenous injected into BALB/cByJNarl male mice. The endotoxin-treated mice were divided into prevention (anticipatory SMF exposed) and treatment (SMF exposed after challenge of LPS) groups. Static magnetic field with a flux intensity of 4000 G was used as an endotoxicity inhibitor. Mice without SMF exposure were set as control group. The survival rates in each group of the samples were calculated for evaluating the endotoxin tolerance capability of SMF. Our results indicated that the survival rate of the tested mice was significantly increased due to SMF exposure in advance for 2 hours before LPS challenge. When the mice were challenged with LPS, the 48 hours survival rate is 16.67±8.08%, which is significantly lower than the survival rate of the mice with 48 hours SMF pre-exposure, 42.86±8.66% (p<0.05). At 48 hours post LPS injection, all the survival mice recovered their health no matter the mice accepted SMF exposure or not. Based on these results, we concluded that further advanced researches on static magnetic fields in inhibiting LPS-induced excessive immune reaction is valuable.