Development of bio-chips for cellular-membrane-protein detection- continuous impedance measurement by ITO di-electrode

• Main Authors: Chun-Yen Lin, 林君彥
• Other Authors: Haw-Ming Huang
• Format: Others
• Language: zh-TW
• Published: 2007
• Online Access: http://ndltd.ncl.edu.tw/handle/48008952282335743678

碩士 === 臺北醫學大學 === 口腔科學研究所 === 95 === Membrane proteins, about 30% of the total proteins, become the major research topics of proteomic projects. There were many techniques were proposed for membrane proteins investigations. However, a method for continuous and long-term protein detection is still unavailable. Accordingly, in this study, we developed an ITO-based biochip for non-destructive membrane protein detection. In our study, when MG-63 cells were seeded on the ITO-based glass chips, a liner relationship was found when comparing with MTT assay (R2=0.9883, p<0.005). Additionally, when Integrin β1 membrane protein were taken into account, there exist a liner relationship between cell impedance and immuno-fluorescence intensity (R2=0.9178, p<0.005). In conclusion, we developed a novel ITO biochip for non-destructive membrane protein detection, and provided some preliminary results for future applications. It will be a useful tool for drugs discovery and cancer cells testing. The results obtained from this study can be a useful reference for future advance investigations on membrane-proteins detection.