Effects of diallyl sulfide and diallyl disulfide on angiogenesis and angiogenesis-related factors

• Main Authors: Sin-Chien Chou, 周歆倩
• Other Authors: 陳玉華
• Format: Others
• Language: zh-TW
• Published: 2007
• Online Access: http://ndltd.ncl.edu.tw/handle/30449673957048643353

碩士 === 臺北醫學大學 === 保健營養學研究所 === 95 === This study was aimed at studying the effects of diallyl sulfide (DAS) and diallyl disulfide (DADS), organosulfur compounds derived form garlic (Allium sativum L.), on the process of angiogenesis. In cultured cells, DAS (2.5~7.5 μM) and DADS (0.05~0.5 μM) enhanced tube formation of EA hy 926 cells, and which was associated with up-regulated expression of pro-angiogenic factors, including VEGF, IL-8 and activated MMP-2 (p<0.05). On the other hand, DAS, DADS, as well as combination of DAS and DADS (0.5+0.005 μM, 1+0.01 μM) inhibited VEGF-stimulated tube formation and activated MMP-2 expression, but increased IL-8 production in EA hy 926 cells (p<0.05). In animals, pathological angiogenesis were induced by subcutaneously injection of 1 μg/ml bFGF-contained matrigel followed by daily ip. injection of DAS (12.5 mg/kg), DADS (50 mg/kg) and DAS+DADS for 10 days. The results showed that DAS, DADS and DAS+DADS mixture did not significantly suppress bFGF-induced hemoglobin contents in matrigels. In summary, DAS and DADS enhanced tube formation under a basal state, but suppressed tube formation in VEGF-stimulated pathological condition, indicating the potential roles of garlic in promoting wound healing as well as in inhibiting carcinogenesis. key words: garlic organosulfur compounds, angiogenesis, wound healing, carcinogenesis