The modulation of purple sweet potato leaves intervention on oxidative stress, cytokines and heat shock protein expression after moderate-intensive exercise

• Main Authors: Ying-Fen Huang, 黃萾芬
• Other Authors: Jen-Fang Liu
• Format: Others
• Language: zh-TW
• Published: 2007
• Online Access: http://ndltd.ncl.edu.tw/handle/40299621782078067492

碩士 === 臺北醫學大學 === 保健營養學系 === 95 === The aim of the study was to investigate the effect of high polyphenol diet intervention on the exercise-induced oxidative stress, cytokine excretion and the expression of heat shock protein 72 (HSP72). Twelve non-trained male subjects participated in two-step dietary intervention period. The study was cross-over design, each subject was given high (purple sweet potato leaves, PSL) or low (β-carotene, BC) polyphenol diets for 7 days, respectively. After 14 days of wash-out period, the diet was cross-over. After each dietary intervention period, the subjects ran on a treadmill for 60 minutes at 70% of their peak oxygen consumption. Blood and 24-hr urine were collected for the measurement of muscle damage, oxidative stress, antioxidant status and repaire system parameter. The results indicated that higher plasma CK (creatine kinase), 8-iso PGF2α (8-iso prostaglandin-F2α), TBARS (thiobarbituric acid reactive substances), TNF-α (tumor necrosis factor-α) were found after exercise performing (p<0.05). Also, it showed significant increase in plasma FRAP (Ferric-reducing antioxidant power), vitamin C and HSP70 protein expression (p<0.05). After 7 days of PSL diet intervention, plasma polyphenol content, FRAP and vitamin E significantly increased (p<0.05). In addtion, plasma TBARS and TNF-α had decreased after one bout of moderate-intensity exercise with PSL diet intervention (p<0.05). However, there was no significant difference in HSP70 level between PSL diet and BC diet. The results indicated that PSL diet intervention can increase total antioxidant potency in body and decrease the oxidative stress and cytokine secretion after moderate-intensity exercise, however there was no effect on HSP70 protein expression.