| Summary: | 碩士 === 臺北醫學大學 === 醫學檢驗生物技術學研究所 === 95 === Clinical studies have shown that after oral administration of a therapeutic dose of glucosamine HCl, a low blood concentration of glucosamine can be achieved, but it does work to diminish pain and repair the cartilage for osteoarthritic patients. Although in vitro data cannot be extrapolated to pathophysiological conditions in humans, our data suggest the effects of action by which glucosamine HCl could modulate IL-1β induced MMPs expression by chondrosarcoma SW1353 cells. The data shows high concentration of glucosamine HCl can reduce the mRNA and protein expression of MMPs which induced by low concentration of IL-1β; low concentration of glucosamine HCl can reduced the inflammatory response up-regulated by high concentration of IL-1β. The data is to comform to clinical application. Besides, we compare the different effects under normoxia and hypoxia to draw on the real condition in human cartilage. HIF-1α protein level would be up-regulated under hypoxia, and furthermore, the level of MMPs protein expression that induced by IL-1β was lower under hypoxia condition. The inhibition of MMPs by glucosamine HCl was more in apparent. We also found GLUT-1 protein expression would up-regulated under IL-1β and hypoxia treatment by chondrocytes. To sum up the results, we suggested HIF-1α and GLUT-1 may be the important mechanisms for therapeutic of glucosamine HCl on osteoarthritis.
|
|---|
