Effect of submerged conditions on the mycelia and polysaccharides of Antrodia cinnamomea

• Main Authors: Hsin-Chen Wang, 王馨晨
• Other Authors: Wen-Yi Yen
• Format: Others
• Language: zh-TW
• Published: 2007
• Online Access: http://ndltd.ncl.edu.tw/handle/54062205117877588542

碩士 === 東海大學 === 食品科學系 === 95 === Abstract Antrodia cinnamomea is an important Chinese medicinal fungus , and is an unique species only grown in Taiwan. It has many functional properties in human body, especially for promoting immune system and protecting liver. As is growing slowly in the wild, supply of A. cinnamomea with stable product quality at reasonable price is in high demand. Research on the methods of artificial cultivation for this fungus is necessary. Our research is interest in attempting to utilize cheaper fermentation substrates for production of this organism, with emphasis on the productivity and quality of the microbial polysaccharides. This study was carried out by submerged fermentation of A. cinnamomea. Culture conditions which including different carbon and nitrogen sources, and different initial pH of fermentation medium in shake flasks. Effects of aeration and stirring in the fermentor were also investigated. The shake flask studies showed that using of corn starch and peptone combination as carbon and nitrogen sources had the best results in production of biomass, the contents of exo-polysaccharide (EPS) and intra-polysaccharide (IPS), with a concentration of 1.20 mg/ml, 17.48 mg/ml, 0.22 mg/ml, respectively in the fermentation broth. The content of β-1,3-glucan in EPS and IPS were 8.80 μg/ml LE, 1.12 μg/ml LE respectively, and had a molecular weight of 3.76×105 Dalton for EPS. Maximum results were obtained from the medium containing substrate concentration of 2% corn starch and unadjusted initial pH, where the biomass, EPS and IPS contents were 0.85 mg/ml, 14.02 mg/ml, 0.07 mg/ml, respectively, as compared to 1% and 3% starch and initial pH at 3, 4, 5 and 6. And β-1,3-glucan of EPS and IPS are 11.06 μg/ml LE and 2.54 μg/ml LE, respectively. The results from a 5 liter stirred tank fermentor ran with three different culture conditions showed that it was most suitable at 100 rpm, aeration rate 1.0 vvm throughout the fermentation period, with regard to production and quality of the polysaccharides. EPS and IPS were 22.06 mg/ml, 0.28 mg/ml, respectively. The β-1,3-glucan of EPS and IPS were 21.81 μg/ml LE and 2.92 μg/ml LE. The exo-polysaccharides had molecular weight of 1.48×104 Dalton.