Deducing the change of transcription regulatory activity on cell cycle related genes in liver cancer by modified network component analysis method

• Main Authors: Chun-Chih, Chen, 陳俊志
• Other Authors: Hu je mei
• Format: Others
• Language: zh-TW
• Published: 2007
• Online Access: http://ndltd.ncl.edu.tw/handle/80181517226239696123

碩士 === 亞洲大學 === 生物資訊學系碩士班 === 95 === Cancer is a complex disease. One of the main properties of cancer cells is their increased and deregulated proliferation rate, which is normally strictly regulated by cell cycle. Abnormalities in the cell cycle related genes can be monitored by the change of their expression profile. It is known that the expression level of a gene is the output of complex transcriptional regulatory systems. Uncover the regulatory activity of transcription factors can help to build the gene regulatory network and understand the nature of carcinogenesis. In this study, a modified network component analysis method was applied to normalized microarray data from 48 patients with liver cancer to deduce and compare the hidden transcriptional regulatory strength of 140 transcription factors on 506 cell cycle-related genes in tumor and non-tumor tissue,respectively. The results demonstrated that the regulatory strength of several transcription factors to cell cycle related genes, such as ATF1 to RC3; MYB to SRD5A1, MAN1A2, HIST2H2BE, FLJ22313, GCSH, ABCC2 and MCM6; APEX1 to HSPA1L; HOXA5 to HIST1H2AC etc..., was strongly changed in tumor tissue. These data could be helpful to understand the change of gene regulatory pathway during oncogenesis.