Effect of herbal proceeding on the bioactivities of Heshouwu(Polygomum multiflorum Thunb.)and Wuweizi (Schisandra chinensis Baill.)

• Main Authors: Shu-yan Ciou, 邱淑燕
• Other Authors: Yi-min Liou
• Format: Others
• Language: zh-TW
• Published: 2007
• Online Access: http://ndltd.ncl.edu.tw/handle/41992692959897845590

碩士 === 大仁科技大學 === 製藥科技研究所 === 95 === Aim The traditional Chinese medicinal materials tuber of Heshouwu and Wuweizi, because compositions are complicated, and more than results of a medicine, so need could reach the anticipated result through proper treatment. But it is treated that the medicine passes different process of preparing Chinese medicine, chemical composition and physics and chemistry nature included , will often produce the change. Time-consuming again process again process of preparing Chinese medicine law that the looks relatively takes a lot of work in the tradition, the modern and scientific process of preparing Chinese medicine law utilizes the vapour pressure cooker, can be in charge of accusing of for concocting the temperature and time in the course. Direct against the change of the effective composition, cooperate with pharmacology to assuare that test the result of incomes with liver, assess the traditional process of preparing Chinese medicine that is applied to Heshouwu and Wuweizi and can be replaced with modern and scientific method in this experiment. Method Research Heshouwu and its index composition after after the tradition is concocted or process of preparing Chinese medicine of the high-temperature high pressure　of　Wuweizi　include　two　styrene　2　,　3　,　5,　4-Tetrahydroxy-stilbene-2-O-glucosid　osid　emodin,　the　Wuweizi　is deoxyschizandrin, gomisin A , schizandrin second. Whether will change to some extent because the ones that concoct the law are different with the acute toxic security of assessing the Heshouwu and Wuweizi of test at the same time. Carry on long period of time experiment further, is it observe two await measuring crude drags if anti-oxidant ferment is it oxidize the hydrogen to pass among the liver to animal used as test before and after not concocting tocatalase and superoxide dismutase influence of the activation. In addition observe the animal used as test in the liver cell’s hormone transforming growth factor, interleukin-1, nterleukin-2 and hepatocytes growth factor, cooperate with pathology to cut the Result of incomes into slices, can explain and await measuring the crude drags and can originally improve the carbon tetrachloride the liver caused is damaged and liver fibre function will have different difference because the ones that concoct the law are different. Results & Discussion The Heshouwu before and after concocting little mouse 15g/kg individually in oral way separately or the Wuweizi, when 72 was a child, found that the little experimental mouse has no death, can know that there is not obvious acute toxicity in this Heshouwu and Wuweizi . After feeding the Heshouwu or eight weeks of process of preparing Chinese medicine product of the fruit of Chinese magnoliavine which eats the big different method incomes of mouse in succession, find that gives their liver function inspections after medicines for a long time, as regards liver function index, it is in charge of feeding the carbon tetrachloride but feed its plasma glutamic-oxalacetictransaminase of group that eats the normal diet taking orally (GOT) and glutamic-pyruvic transaminase (GPT) activation is higher than controlling the group apparently, it leads to the fact the liver cell to release ferment GOT and GPT in the liver in the plasma downright badly that the liver showing the carbon tetrachloride and leading is damaged , cause the rising of the liver function index in the plasma , Add silymarin and low dosage and high dosage behind catching producting or Heshouwu and Wuweizi to concoct product among coming out mouse diet, can reduce the liver that the carbon tetrachloride causes to damage apparently , and then slow down the situation that GOT and GPT activation rise in the plasma . Can know the result of study of the experiment the Heshouwu and Wuweizi can slow down the situation that GOT and GPT activation rise in the plasma , and reach the protection result of the liver, among them is superior to the product of growing by concocting products , concoct the Heshouwu superior to the traditional process of preparing Chinese medicine law with the process of preparing Chinese medicine law that the high temperature presses in products and Wuweizi, the active results of GOT and GPT are best while slowing down plasma; All have function which reduces the fat of blood to check triacylglycerols , cholesterol value too in the blood fat. Check blood urea nitrogen and creatinine and find that its value is all in the standard in the kidney function. Is it under Heshouwu and Wuweizi before and after take for a long time , have function protected to the liver to concoct, have function reduced but not influence the kidney function part on the fat of blood too, pharmacology machine that pharmacology protects liver function is transferred to the respect, hydrogen that the Wuweizi and Heshouwu of crude drags among them will increase the anti-oxidant ferment in the liver and catalase, superoxide dismutase situation that the activation rises, and then gat the result of protecting liver in pharmacology; The Wuweizi and Heshouwu of traditional Chinese medicinal materials will reduce the cell hormone in the liver and transforming growth factor, interleukin-1 , interleukin-2, and then suppress liver damaging and liver fibre caused of carbon tetrachloride. The result studied according to the experiment , the ones that are assuared by pharmacology and tested biochemical value of serum , anti-oxidant ferment and cell’s hormone and studied with liver are under the card, it is more apparent than not to concoct the result to confirm the readitional Chinese medicinal materials Heshouwu knotweed and result after concocting of Wuweizi , so the course concocted has its necessity, it is studied its function of process of preparing Chinese medicine law that learns to press in high temperature and superior to the traditional concocting more method that then concoct the relatively having of the law, and can press the law of concocting to replace the traditional process of preparing Chinese medicine law with the high temperature that is improved among them, not only can concoct necessary time sparingly and can be applied to the pharmaceutical factory and produced a large amount of .