Genetic Diversity of Aristolochia plant in Taiwan

• Main Authors: Szu-Lin Haung, 黃思霖
• Other Authors: Che-feng Lu
• Format: Others
• Language: zh-TW
• Published: 2007
• Online Access: http://ndltd.ncl.edu.tw/handle/61711300441935244734

碩士 === 大仁科技大學 === 製藥科技研究所 === 95 === Plants of the genus Aristolochia (Aristolochiaceae) are important Chinese traditional medicine. Six species, i.e. Aristolochia cucurbitifolia, A. heterophylla, A. kaempferi, A. zollingeriana, A. foveolata, and A. shimadai are known to be distributed in Taiwan. Taxonomy and systematics of Aristolochia have been analyed based on morphological characters, chemical constituents, and karyotypes during the last decade. Nevertheless, controversies still remain to the present. In this study, we analyzed sequence differences of the internal transcribed spacers (ITSs) and atpB-rbcL sequences from six Aristolochia species and reconstructed the phylogenetic relationships. The lengths of the ITS sequences varied from 661 bp (A. zollingeriana) to 690 bp (A. shimadai). All G+C contents approximate 63.7%. The lengths of the atpB-rbcL sequences major are 706 bp except 750 bp (A. foveolata) and 733 bp (A. zollingeriana). All A+T contents approximate 69.5%. Genetic distances ranged from 0.007 (A. kaempferi vs. A. shimadai) to 0.609 (A. zollingeriana vs. A. heterophylla) on ITS. Genetic distances ranged from 0.002 (A. kaempferi vs. A. heterophylla) to 0.057 (A. cucurbitifolia vs. A. foveolata and A. shimadai vs. A. foveolata) on atpB-rbcL sequences. The phylogenetic tree generated with the neighbor-joining method and maximum parsimony method all revealed that Taiwan’s Aristolochia is divided into two groups. The first group includes A. zollingeriana and A. foveolata, while A. cucurbitifolia, A. heterophylla, A. kaempferi and A. shimadai were clustered in the other group.