| Summary: | 碩士 === 國立臺灣科技大學 === 化學工程系 === 95 === Chitosan was used in this study to fabricate asymmetric scaffolds for the guided tissue regeneration (GTR) in the treatment of periodontal diseases. With the two-step phase separation, the lacy structure was formed by liquid-liquid demixing on the top layer of scaffolds, where the pore size was about 1 um. On the other hand, the pores on the bottom layer were cellular and ranged from 80 to 120um, which were formed by the formation of ice crystals. From the culture of osteoblastic cells, the tissue integration, cell occlusivity and space-making effect of the novel chitosan scaffold were all excellent for four months; meanwhile, the transport of tissue fluid would not be hindered by the top layer with small pores. Compared with commercial PLLA GTR membranes and chitosan scaffolds with dense skin, the asymmetric chitosan scaffold developed in this research significantly expressed better biocompatibility and osteoconductivity. The anti-bacterial activity of the asymmetric chitosan scaffold would also last at least for four months. From above results, the asymmetric chitosan scaffold was proved to be a potential GTR membrane for the regeneration of alveolar bones.
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