| Summary: | 碩士 === 國立臺灣大學 === 醫學工程學研究所 === 95 === In spinal cord injury, activated astrocytes migrated to the injury site and secreted the extracellular matrix (ECM), called scar tissue. The scar tissue impeded the re-growth of neurons and axon and inhibited nerve regeneration. It had proved that Chondroitinase ABC can digest the polysaccharide of proteoglycan in scar tissue, promoted the re-growth of neuron, axon and behavior recovery. In order to improve the stability of chondroitinase ABC, and maintain the activity of chondroitinase ABC, the feasibility of using immobilized chondroitinase ABC on nerve conduit and encapsulated chondroitinase ABC in PDLLA microsphere were studied further in this work.
Chitosan and gelatin were used to fabricate the conduit with different pore diameter. The pore diameter of chitosan conduit was 100-160 μm, and the pore diameter of chitosan/gelatin conduit was 20-40μm. Chondroitinase ABC was immobilized on surface of the conduit by ionic binding and covalent binding. The activity of chondroitinase ABC immobilized on chitosan conduit by ionic binding was 0.07 U/mg, and the activity recovery was 48% after 48 hours. The stability of the immobilized chondroitinase ABC was significantly improved. PDLLA microsphere was fabricated through double emulsion and controlled release of chondroitinase ABC. Activity of chondroitinas ABC released from microsphere without stabilizer was lower than 0.0026 U/ml during seven days. The results indicated that the microsphere made by double emulsion and added the optimal stabizer can increase the efficacy of the control release and the duration of chondroitinase ABC activity.
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