Effect of Narrow Bandwidth Infrared Radiation on Mungbean Growth and Gene Expression

• Main Authors: Chi-Feng Chen, 陳季鋒
• Other Authors: Si-Chen Lee
• Format: Others
• Language: en_US
• Published: 2007
• Online Access: http://ndltd.ncl.edu.tw/handle/49480793485520687857

碩士 === 國立臺灣大學 === 電機工程學研究所 === 95 === The purpose of this study is to design a narrow bandwidth, high power infrared light source and investigate the effect of plants exposed to these infrared irradiation with specific waveband during seed germination and seedling development of mungbean. This infrared emitter can be achieved by heating the triple layer structure which consists of an SiO2 layer between two Ag films on a Si substrate. The top Ag layer is perforated by periodic holes, the emission wavelength can be altered by changing the lattice constant and diameter of the hole arrays. Five plasmonic thermal emitters with different emission peak wavelength, which are 4.81, 4.49, 3.90, 3.62 and 3.17 μm, were designed and fabricated. The full width at half maximum (FWHM) of these emitters are 0.6, 0.68, 0.92, 0.7 and 0.48 μm, respectively. After the 4 days exposed to different wavelength of infrared radiation treatment, the hypocotyl length of mungbean is measured and the Northern blot analysis is applied to observe the gene expression pattern of chosen genes GIR1, CHS and RbcS. The experimental results revealed that the infrared of wavelength 4.81 and 3.62 μm do not affect the growth of mungbean, neither the elongation of hypocotyl length nor gene expression patterns. The infrared of wavelength 4.49 and 3.90 μm inhibits the elongation of hypocotyl length by 39.81 and 20.09%, respectively. From the gene expression pattern, we can also find the expressions of GIR1 are altered by infrared treatment. The 3.17 μm infrared irradiation do not affect the hypocotyl length of mungbean, but the gene expression of GIR1 is reduced by 24.03% compared to the dark control group. The gene expression of CHS and RbcS do not have significant change in this experiment, showing that the gene expressions of these two genes are not influenced by infrared exposure.