Construction of an inducible transposable element to terminate selectable marker gene

• Main Authors: Hung-Kuang Tai, 戴宏光
• Other Authors: 常玉強
• Format: Others
• Language: zh-TW
• Published: 2007
• Online Access: http://ndltd.ncl.edu.tw/handle/05230565798925670641

碩士 === 臺灣大學 === 農藝學研究所 === 95 === Selectable marker genes (SMGs) are often necessary during the process of plant transformation, but unnecessary once transgenic plants have been obtained. Besides, SMGs is involved in biosafety. Therefore, in this work, an inducible transposable element was constructed to terminate the selectable marker gene：modified EPSPS gene. Firstly, the 3’ end of the Ac was inserted in the first intron of the modified rice EPSPS gene, which triggered by the nos promoter. Secondly, The 3’ end of the Ac was inserted in the fourth intron of the inducible Ac transposase gene trigged by PR-1a promoter. Thus, this inducible transposable element contains not only the exon E of Ac transposase gene but also the first exon of the modified EPSPS gene and its promoter. This construct, which termed as Cpt5ne3, was introduced into rice, tobacco, and arabidopsis. The modified EPSPS gene can be transcribed normally in all three species and allow the transgenic rice and arabidopsis to be Glyphosate-resistant. Also, the transposase gene is transcribed correctly. Therefore, it is expected that transposition event can be induced by salicylic acid