| Summary: | 碩士 === 國立臺灣大學 === 動物科學技術學研究所 === 95 === The purpose of the experiment was to investigate the physiological parameters of bone growth and to establish the avian tibial chondrocyte culture system. Native chicken, broiler, mule duck and Pekin duck were used as the experiment materials. In order to understand the tibial development process in the embryonic stage, type II collagen and glycosaminoglycans were assayed for studying of different sources morphology of chondrocyte in vivo. Growth plate from tibial cartilages of chicken embryos(12 days of incubation), Pekin duck embryos(16 days of incubation) and mule duck embryos(18 days of incubation) were cultured in vitro for 15 days. Alkaline phosphatase (ALP), lactate dehydrogenase (LDH) activities and type II collagen contents in chondrocytes were measured every 3 days during the culturing period. Alizarin red S was used to measure the degree of mineralization. The results showed that we could not only recognize meat-type poultry tibia growth characteristics in the embryonic post-stage, but also understand the secretion of extracellular matrix. We found that meat-type chicken group had a significant longer tibia length and the amount of glycosaminoglycan in tibia at day14-15 after incubation. We also found that in meat-type duck group at day 23-24 after incubation in vitro experiment, we found ALP activity of native chickens were higher than that of broilers from day 6 to day 12（P<0.05）after culturing. The ALP activity of Pekin ducks were higher than those of mule ducks (437.67 vs. 265.81 U/mg), and ALP activity of native chickens were higher than those of broilers (390.13 vs. 171.41 U/mg) in chondrocytes. The protein concentration of type II collagen in ducks was higher than that of chickens in chondrocytes. The results revealed that the duck chondrocyte had earlier characteristics of mineralization in embryo stage than those of chickens. The established culture system may be used for the study of factors that affect the chondrocytes growth in poultry.
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