| Summary: | 碩士 === 國立臺灣大學 === 食品科技研究所 === 95 === Cellulose is the most abundant polysaccharide on the earth and is generally applied to foods, fuel, biomaterials and energy. Degradation of cellulose to glucose, which is then fermented to ethanol, is one of the hottest issues in the past decade. The objective of this study is to investigate the effect of medium milling on the kinetics of enzymatic cellulose hydrolysis. The volume average particle size of raw cellulose was reduced from 25.52 μm to 1 ~ 10 μm. Different concentration of cellulose and milling time produced cellulose with various average particle sizes for further enzymatic hydrolysis. Kinetics of glucose and cellobiose production was applied to explore the effect of milling on cellulose hydrolysis.
15 g or 30 g cellulose and 500 mL deionized water (i.e. 3 % or 7 %) were milled by milling medium, YTZ (yttria-stabilized tetragonal zirconia) beads with average diameter 0.3 mm. After 0, 15, 60 and 120 min milling, volume average particle size of 3 % cellulose was 25.52 μm, 5.54 μm, 0.85 μm and 0.78 μm, respectively, and crystallinity index was 94.4 %, 87.5 %, 74.3 % and 77.0 %, respectively; while for 7 % cellulose, volume average particle size was 25.52 μm, 6.08 μm, 2.66 μm and 2.07
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