Fabrication of micropatterned polyelectrolyte multilayers for controlling cell adhesion

• Main Authors: Tsung-Yao Chang, 張琮瑤
• Other Authors: Wei-Bor Tsai
• Format: Others
• Language: zh-TW
• Published: 2007
• Online Access: http://ndltd.ncl.edu.tw/handle/98415094626920358785

碩士 === 臺灣大學 === 化學工程學研究所 === 95 === The topography of micropatterned polyelectrolyte multilayers can be controlled in molecule scale and is extremely versatile and useful for in vitro studies of cell behavior or biomedical devices. Polyacrylamide (PAAm) and poly(acrylic acid) (PAA) can be assembled to form polyelectrolyte multilayers via hydrogen-bonding interactions at low pH. The PAA/PAAm films can be rinsed away with alkaline water. Azides, a photo-reactive molecule, can crosslink neighbor hydrocarbon chains under UV irradiation. First, 4-azidoaniline hydrochloride was grafted on PAA to form PAAz. Then, PAA/PAAm films were fabricated on glass slide for 4 bilayers, followed by deposition of one PAAz/PAAm bilayer before further build-up of PAA/PAAm films. Micropatterned polyelectrolyte multilayers with different numbers of bilayers were fabricated by UV exposure of the multilayers films through photomasks with 20 μm or 50 μm periods of parallel lanes. Poly-L-lysine was adsorbed on micropatterned films before in vitro cell experiments. We found MG63 and L929 cells adhered to ditch region and regularly arranged along the direction of ditch. On the other hand, PC12 cells extended neurites parallel to the striped pattern, but adhered on both ditch and ridge regions. Cell alignment on the micropatterned films with 50 μm periods was better than 20μm periods. These works successfully developed a technique utilizing polyelectrolyte multilayers and photolithography technology to prepare micropatterned substrata with controlled surface chemistry for modulation of cell adhesion.