| Summary: | 碩士 === 國立臺灣大學 === 分子與細胞生物學研究所 === 95 === All-trans retinoic acid (ATRA) and Am80, natural and synthetic derivatives of Vitamin A, have been used in oncology for many years and are useful for inducing HL-60 to differentiate to granulocyte. In this study, we showed that TGF-β signaling pathway is the most perturbed and involved in differentiation process using microarray and bioinformatics approach. With time-series gene expression profiles measured by the quantitative real-time PCR, we constructed a gene network of ATRA- and Am80-induced human leukemia cell line HL-60 differentiation. The results from comparative analysis of target genes indicated that this gene network was regulated by some candidate microRNAs. MicroRNAs are non-coding, single-stranded RNAs of 20 ~ 25 nucloetides that execute a gene regulator function responsible for cell growth, cell differentiation, disease, and cancer. In our results, the expression of miR-96 and miR-107 were significantly induced by ATRA and Am80. In summary, our results showed that miR-96 and miR-107 seem to be the regulators involved in the gene network of leukemia cell differentiation.
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