The Discovery of A High Agarolytic Bacterium Alcaligenes sp. Strain Yen
碩士 === 國立臺東大學 === 生命科學研究所 === 95 === An aerobic, Gram negative extracellular agarase producing bacillus was discovered in Taitung, Taiwan. This bacterium was identified as Alcaligenes sp. strain Yen (AY744384). It has a plasmid with 18,400 bp and had been succesfully transformed into competent E. co...
| Main Authors: | , |
|---|---|
| Other Authors: | |
| Format: | Others |
| Language: | zh-TW |
| Published: |
2007
|
| Online Access: | http://ndltd.ncl.edu.tw/handle/vq4s26 |
| id |
ndltd-TW-095NTTU0105004 |
|---|---|
| record_format |
oai_dc |
| spelling |
ndltd-TW-095NTTU01050042019-09-19T03:29:58Z http://ndltd.ncl.edu.tw/handle/vq4s26 The Discovery of A High Agarolytic Bacterium Alcaligenes sp. Strain Yen 高活性洋菜分解菌Alcaligenes sp. Strain Yen之發現 YANG, HUEI-CHUN 楊惠春 碩士 國立臺東大學 生命科學研究所 95 An aerobic, Gram negative extracellular agarase producing bacillus was discovered in Taitung, Taiwan. This bacterium was identified as Alcaligenes sp. strain Yen (AY744384). It has a plasmid with 18,400 bp and had been succesfully transformed into competent E. coli HB 101 cells. Positive colonies were visualized forming pits on the modified C-10 agar plate. The enzyme was purified from culture supernatant by ammonium sulfate precipitation and gel filtration column chromatography method. The optimal activity for purified enzyme was at pH 7.5 and at temperature 45 ℃. The enzyme had a specific agarase activity of 3,200 U/mg. Estimation of the agarase molecular mass by SDS-PAGE was 101 kDa. The agarolytic bacterium have the highest agarase activity among all previous reported studies. LI, YAN 李炎 2007 學位論文 ; thesis 32 zh-TW |
| collection |
NDLTD |
| language |
zh-TW |
| format |
Others
|
| sources |
NDLTD |
| description |
碩士 === 國立臺東大學 === 生命科學研究所 === 95 === An aerobic, Gram negative extracellular agarase producing bacillus was discovered in Taitung, Taiwan. This bacterium was identified as Alcaligenes sp. strain Yen (AY744384). It has a plasmid with 18,400 bp and had been succesfully transformed into competent E. coli HB 101 cells. Positive colonies were visualized forming pits on the modified C-10 agar plate. The enzyme was purified from culture supernatant by ammonium sulfate precipitation and gel filtration column chromatography method. The optimal activity for purified enzyme was at pH 7.5 and at temperature 45 ℃. The enzyme had a specific agarase activity of 3,200 U/mg. Estimation of the agarase molecular mass by SDS-PAGE was 101 kDa. The agarolytic bacterium have the highest agarase activity among all previous reported studies.
|
| author2 |
LI, YAN |
| author_facet |
LI, YAN YANG, HUEI-CHUN 楊惠春 |
| author |
YANG, HUEI-CHUN 楊惠春 |
| spellingShingle |
YANG, HUEI-CHUN 楊惠春 The Discovery of A High Agarolytic Bacterium Alcaligenes sp. Strain Yen |
| author_sort |
YANG, HUEI-CHUN |
| title |
The Discovery of A High Agarolytic Bacterium Alcaligenes sp. Strain Yen |
| title_short |
The Discovery of A High Agarolytic Bacterium Alcaligenes sp. Strain Yen |
| title_full |
The Discovery of A High Agarolytic Bacterium Alcaligenes sp. Strain Yen |
| title_fullStr |
The Discovery of A High Agarolytic Bacterium Alcaligenes sp. Strain Yen |
| title_full_unstemmed |
The Discovery of A High Agarolytic Bacterium Alcaligenes sp. Strain Yen |
| title_sort |
discovery of a high agarolytic bacterium alcaligenes sp. strain yen |
| publishDate |
2007 |
| url |
http://ndltd.ncl.edu.tw/handle/vq4s26 |
| work_keys_str_mv |
AT yanghueichun thediscoveryofahighagarolyticbacteriumalcaligenesspstrainyen AT yánghuìchūn thediscoveryofahighagarolyticbacteriumalcaligenesspstrainyen AT yanghueichun gāohuóxìngyángcàifēnjiějūnalcaligenesspstrainyenzhīfāxiàn AT yánghuìchūn gāohuóxìngyángcàifēnjiějūnalcaligenesspstrainyenzhīfāxiàn AT yanghueichun discoveryofahighagarolyticbacteriumalcaligenesspstrainyen AT yánghuìchūn discoveryofahighagarolyticbacteriumalcaligenesspstrainyen |
| _version_ |
1719252046807302144 |