Variations of Cell Membrane under the External Force Modulation

• Main Authors: Hung-Jhang Jian, 簡宏彰
• Other Authors: Chih-Wei Wu
• Format: Others
• Language: zh-TW
• Published: 2007
• Online Access: http://ndltd.ncl.edu.tw/handle/90179298567630703150

碩士 === 國立臺灣海洋大學 === 機械與機電工程學系 === 95 === The study of cell mechanics is crucial for many cellular functions, such as cell growth, differentiation, migration, gene expression and apoptosis. Using external manipulation technique to induce cell deformation is the major method for the studies of cell mechanics. Cell membranes play important roles on cell motion and morphology maintenance, but most of current techniques cannot directly and rapidly obtain the topography variation of cell membrane. The mechanism of cell membrane variation under the external force is not clear yet. In this thesis, we combine non-interferometric widefield optical profilometry (NIWOP) with permanent magnetic microneedle (PMM) to observe the membrane deformation under external force manipulation. We use a fibronectin coated paramagnetic bead to attach to the cell membrane and use the PMM to apply external force to cell membrane via the bead. We change the magnetic flux of magnet, distance from the paramagnetic bead to the magnetic microneedle, and the tip shape of magnetic microneedle to modulate the strength of external force. NIWOP can rapidly and non-intrusively obtain membrane topography with nanometer depth resolution. Therefore, we can measure the membrane deformation precisely. According to the experimental results, we reach the following conclusions: First, the total force from the external magnetic field and the cytoskeletons of the cell determines the motion of the paramagetic bead on the cell membrane. Second, the motion of the paramagnetic bead on the membrane causes the membrane deformation when it is under the action of external force. Finally, the motion of paramagnetic bead on the membrane will be constrained by the membrane topography when the paramagnetic bead was not attracted by the magnetic field.