Development of an effective Helicobacter pylori-specific DNA vaccine

• Main Authors: Yun-Han Chu, 朱韻涵
• Other Authors: Chang-Jer Wu
• Format: Others
• Language: zh-TW
• Published: 2007
• Online Access: http://ndltd.ncl.edu.tw/handle/85714797051950989891

碩士 === 國立臺灣海洋大學 === 食品科學系 === 95 === A strong correlation between Helicobacter pylori (H. pylori) and many gastrointestinal diseases including: gastritis, peptic ulcer and even gastric cancer are reported by Warren and Marshall in 1983. The presence of H. pylori is also strongly associated with delayed healing of ulcer recurrence. Eradication of H. pylori makes the dream of cured peptic ulcer diseases come true. However, the pathogenesis of H. pylori-associated diseases is not clear. The urease production is an important criterion for H. pylori to survive in the stomach. An animal model for H. pylori infection is needed to investigate the pathogenesis of H. pylori and the protective efficiency of the anti-H. pylori vaccines. So we will use two different strains of H. pylori, including one standard strain (BCRC 15415) and one clinical strain (HC28), to set up the H. pylori-infected C3H/HeN mice model for this study. We fed 6-8 weeks old C3H/HeN mice by intragastric method with 0.5ml H. pylori (1 x 109 CFU/ml) for three times in a week interval. The results show that H. pylori can infect C3H/HeN mice successfully, and the inflammatory cytokines can also be detected in the gastrointestinal tract. The urease protein is the most accepted and potential vaccine candidate of the many investigated antigens of H. pylori. Therefore, we clone H. pylori urease B gene into the prokaryotic and eukaryotic expression vector, pET-30a and pCJ-3, respectively. Then, we compare the protective effect of the H. pylori DNA vaccine, subunit vaccine and whole-lysate vaccine on the established mice model. We hope to develop an effective Helicobacter pylori DNA vaccine in the mice model.