Optimization of the Production of Cellulomonas sp. Cellulase Using 5-Liter Fermenter and Its Effect on the Hydrolysis of Chlorella

• Main Authors: Chen-I Ju, 陳奕如
• Other Authors: Shann-Tzong Jiang
• Format: Others
• Language: zh-TW
• Published: 2007
• Online Access: http://ndltd.ncl.edu.tw/handle/57159914060621651311

碩士 === 國立臺灣海洋大學 === 食品科學系 === 95 === Chlorella, rich in proteins, peptides, chlorophyll, vitamins and minerals, is a very good material for the production of functional foods. To hydrolyze the cell wall of Chlorella, a cellulase-producing bacterium, Cellulomonas sp. was isolated and used to produce cellulase for the hydrolysis of chlorella cell wall. The cellulase activity could reach 1320 U/ml after 3 day fermentation in a 5-L fermenter at 100% DO and 50 rpm. No significant difference in cellulase activity was observed between those spray-dried with 10 and 15 % dextrin carrier. However, the spray-dried samples were unstable, even stored at 4 oC with vacuum packing, almost 80% activity was lost after 8 wks storage. Contents of proteins, peptides and chlorophyll of both Chlorella powders dried at low temperature and spray drying increased after 3 hr hydrolysis. According to microscopic and SEM observations, Chlorella cell wall was obviously hydrolyzed. The aggregates of chlorella cells after spray-drying could be hydrolyzed into cell individuals with 150 U/ml of cellulase. Increases in the contents of chlorophyll and the xanthophylls after 2 hr hydrolysis with 300U/ml suggested the lysis of chlorella cell walls.