Identification of antigens recognized by anti-DNA and anti-ribosome P protein antibodies using mass spectrometry.

碩士 === 國立臺灣海洋大學 === 生物科技研究所 === 95 === Systemic Lupus Erythematosus is a chronic-immune disease affected many somatic organs such as joints, skin, kidney and et al by auto-antibodies, including anti-DNA and anti-nuclear protein antibodies. Auto-antibodies, 9B6 and 9D7 recognizes ribosomal P protein...

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Main Authors:	Ghien-Hung Chiang, 江建宏
Other Authors:	Shye-Jye Tang
Format:	Others
Language:	zh-TW
Published:	2007
Online Access:	http://ndltd.ncl.edu.tw/handle/99002912575664235146

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spelling	ndltd-TW-095NTOU51110242015-10-13T11:31:39Z http://ndltd.ncl.edu.tw/handle/99002912575664235146 Identification of antigens recognized by anti-DNA and anti-ribosome P protein antibodies using mass spectrometry. 鑑定抗DNA與抗核醣P蛋白質抗體與SLE相關之自體抗原 Ghien-Hung Chiang 江建宏碩士國立臺灣海洋大學生物科技研究所 95 Systemic Lupus Erythematosus is a chronic-immune disease affected many somatic organs such as joints, skin, kidney and et al by auto-antibodies, including anti-DNA and anti-nuclear protein antibodies. Auto-antibodies, 9B6 and 9D7 recognizes ribosomal P protein and DNA, respertively. To identify one cross-reactive antigens of these antibodies, mass spectrometry was performed after immunoprecipitation and SDS-PAGE. 28 candidates of antigens were analysed by LC-MS. Glucose-regulated protein 78 (GRP78), one of candidate, were characterized by co-immunoprecipitation and western blotting using 9B6 antibody. Our results indicated that this strategy may be used to identify the complex recognized by auto-antibodies and to study the pathogenesis of SLE. Shye-Jye Tang 唐世杰 2007 學位論文 ; thesis 90 zh-TW
collection	NDLTD
language	zh-TW
format	Others
sources	NDLTD
description	碩士 === 國立臺灣海洋大學 === 生物科技研究所 === 95 === Systemic Lupus Erythematosus is a chronic-immune disease affected many somatic organs such as joints, skin, kidney and et al by auto-antibodies, including anti-DNA and anti-nuclear protein antibodies. Auto-antibodies, 9B6 and 9D7 recognizes ribosomal P protein and DNA, respertively. To identify one cross-reactive antigens of these antibodies, mass spectrometry was performed after immunoprecipitation and SDS-PAGE. 28 candidates of antigens were analysed by LC-MS. Glucose-regulated protein 78 (GRP78), one of candidate, were characterized by co-immunoprecipitation and western blotting using 9B6 antibody. Our results indicated that this strategy may be used to identify the complex recognized by auto-antibodies and to study the pathogenesis of SLE.
author2	Shye-Jye Tang
author_facet	Shye-Jye Tang Ghien-Hung Chiang 江建宏
author	Ghien-Hung Chiang 江建宏
spellingShingle	Ghien-Hung Chiang 江建宏 Identification of antigens recognized by anti-DNA and anti-ribosome P protein antibodies using mass spectrometry.
author_sort	Ghien-Hung Chiang
title	Identification of antigens recognized by anti-DNA and anti-ribosome P protein antibodies using mass spectrometry.
title_short	Identification of antigens recognized by anti-DNA and anti-ribosome P protein antibodies using mass spectrometry.
title_full	Identification of antigens recognized by anti-DNA and anti-ribosome P protein antibodies using mass spectrometry.
title_fullStr	Identification of antigens recognized by anti-DNA and anti-ribosome P protein antibodies using mass spectrometry.
title_full_unstemmed	Identification of antigens recognized by anti-DNA and anti-ribosome P protein antibodies using mass spectrometry.
title_sort	identification of antigens recognized by anti-dna and anti-ribosome p protein antibodies using mass spectrometry.
publishDate	2007
url	http://ndltd.ncl.edu.tw/handle/99002912575664235146
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Identification of antigens recognized by anti-DNA and anti-ribosome P protein antibodies using mass spectrometry.

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