General reaserch on the resistance of water spinach (ipomoea aquatica) to white rust disease caused by Albugo ipomoeae-aquaticae

• Main Author: 簡君如
• Other Authors: 童武夫
• Format: Others
• Language: zh-TW
• Published: 2007
• Online Access: http://ndltd.ncl.edu.tw/handle/79771471195902390387

碩士 === 國立臺灣師範大學 === 生命科學研究所 === 95 === Water spinach (Ipomoea aquatica Forsk.), an important summer vegetable in Taiwan, is easily infected by Albugo ipomoeae-aquaticae, in the result of white rust disease. By cotyledon inoculation, our result shows ‘white-seed Jwu Yeh’ lines were most strongly resistant to white rust disease. We also surveyed several factors of water spinach to evaluate the association with disease proportion; Such as stomatal density of cotyledon, contents of photosynthetic pigments (chlorophyll a, chlorophyll b, and carotenoids), concentration of glucose and fructose had positive correlation with disease proportion of juvenile stage in non-linear regression. On the other hand, the concentration of sucrose became negative correlation in simple linear regression. The XY scattered graphs of stomatal density, chlorophyll a, chlorophyll b, glucose and fructose with disease proportion were suggested that they exhibited a grouping relationship. Besides, there was multiple regression correlation between six horticultural characteristics and disease severity. From r2, the predictive power of the multiple regression equation would exceed 0.6. We further used Random Amplified Polymorphic DNA (RAPD) to analyze the different banding patterns between one strong resistance line, 2002-026, and other susceptive lines. The random primer O15 produced an unique band (about 1500 bp) which is present only in the lane of DNA amplified from line, 2002-026, but absent in others. We hope the results of our investigation could help in the further research on resistance breeding and defense gene screening.