| Summary: | 碩士 === 國立清華大學 === 化學工程學系 === 95 === A major challenge in the use of gene transfer vectors as therapeutic tools is
controlling vector administration at a desired tissue site. One potential solution is
implanting tissue-engineering constructs loaded with gene transfer vectors such as
viruses for localized transgene delivery. In this work, we conjugated recombinant
adeno-associated virus serotype 2 (rAAV2) to a heparinized small intestinal
submucosa (H-SIS) matrix, which resulted in vector transduction upon cellular
adhesion. H-SIS was prepared by incorporating heparin, the rAAV2 receptor, into SIS
through N-(3-dimethylaminopropyl)-N’-ethylcarbodiimide (EDC) and
N-hydroxysuccinimide (NHS) mediated crosslinking. Incorporated heparin adsorbed
rAAV2 onto the H-SIS matrix for conjugation. Using green fluorescent protein and
��-galactosidase as reporters, we showed that conjugated rAAV2 was active and
capable of mediating transgene delivery in cell culture. Our work provides a unique,
modified tissue substrate H-SIS for rAAV2 binding and transduction, which can be a
useful tool in developing localized gene transfer.
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