Stretching and Deformation of DNA Molecules in Converging-Diverging Microchannels

• Main Authors: Jian-Heng Liou, 劉建亨
• Other Authors: Shou-Shing Hsieh
• Format: Others
• Language: zh-TW
• Published: 2007
• Online Access: http://ndltd.ncl.edu.tw/handle/dd662j

碩士 === 國立中山大學 === 機械與機電工程學系研究所 === 95 === In this study, sharp/gradual converging-diverging microchannels with contraction/ expansion ratio of 4:1/1:4 was designed to generate elongational flow with uniform velocity in the centerline. The λ-DNA stained with YOYO-1 was observed in the flow. MPIV was built to measure the velocity distribution and local strain rate was estimated by MPIV measurements. The deformation and conformation of individual DNA molecules in the flow was visualized with confocal laser scanning microscopy (CLSM). The goal of the present work was to develop a method for stretching DNA molecules, in order to perform analysis of coil-stretch transition of DNA. By measuring dynamic properties and relaxation time of DNA molecules stretched by pressure driven at various flow rate and viscosity, we have shown how one could investigate the influence of hydrodynamic interactions in the case of stretching of DNA molecules.