Selection of disease resistant plantlets of Oryza sativa var. Japonica Taiken 9 induced with culture filtrate of Xanthomonas oryzae pv. oryzae

• Main Authors: Hsu Shin-Yun, 許馨云
• Other Authors: 鄭秋雄
• Format: Others
• Language: zh-TW
• Published: 2007
• Online Access: http://ndltd.ncl.edu.tw/handle/54808613468933670588

碩士 === 國立屏東科技大學 === 植物保護系所 === 95 === The purposes of this study were to select the bacterial leaf blight- resistant plants of rice, to develop the technologies of disease-resistant selection and callus induction of Oryza sativa var. Japonica Taiken 9 through plant tissue culture. In the technique of disease-resistant selection, added 108 cfu/ml X. oryzae pv. oryzae in 100 ml YPDA liquid medium and the culture filtrate of X. oryzae pv. oryzae cultured for 72 hours as the selection agent provided the best selection result. The callus, little than 2 mm in diameter, were treated with the agent, added 1.5 g callus in 20 ml culture filtrate of X. oryzae pv. oryzae 6 days after selection, which could result in the browning index of callus about 72.83-79.93%. In the culture conditions of the callus induction of Oryza sativa var. Japonica Taiken 9, CS-1 medium containing 2 mg/l 2,4-D and 2.8 g/l proline, at 5600-6200 lux light intensity for 45 days had the best result in callus formation. The fresh weight of callus per seed about 0.214 g，and this method could induced nodular callus. In order to select the bacterial leaf blight-resistant plants of rice, using the culture filtrate of X. oryzae pv. oryzae as the selection agent, and the selection method was to select with the crude culture filtrate of X. oryzae pv. oryzae twice (the first and second selection) and then selected with concentrated one fold culture filtrate of X. oryzae pv. oryzae once (the third selection). The resistant callus lines and nonresistant callus lines were 23.21% and 76.79% in the first selection, the second selection were 15.04% and 84.96%, and the third selection were 4.75% and 95.25%. The plantlets regeneration rate of the resistant callus lines and nonresistant callus lines were 1.20% and 8.17% in the first selection, the second selection were 8.24% and 0%, and there were not regenerated any plantlets all of them in the third selection. Inoculated 5 plants regenerated from the resistant callus lines (browning level 0), 35 plants (browning level 1) and 19 plants (browning level 2-3) regenerated from the nonresistant callus lines and 47 plants of Oryza sativa var. Japonica Taiken 9 with X. oryzae pv. oryzae, respectively. The disease-resistant plants obtained from these inoculated plants were 2 plants (40%), 14 plants (48.57%) and 5 plants (26.32%), on the contrary, all the plants of Oryza sativa var. Japonica Taiken 9 were sensitivity to X. oryzae pv. oryzae. Inoculated the self-pollinated progeny (cfXoo-1-1 F1 and cfXoo-1-6 F1) of bacterial leaf blight-resistant plants and Oryza sativa var. Japonica Taiken 9 with X. oryzae pv. oryzae to detect the inheritance of resistibility. The disease-resistant plants obtained from these inoculated plants were 8 plants (42.1%), 15 plants (35.7%), and all the plants of Oryza sativa var. Japonica Taiken 9 with X. oryzae pv. oryzae were sensible.