Micro Flow Cytometer for cell/particle Detection

• Main Authors: Xu Zhe Chun, 許哲淳
• Other Authors: Lung-Ming Fu
• Format: Others
• Language: zh-TW
• Published: 2007
• Online Access: http://ndltd.ncl.edu.tw/handle/06211582545209850095

碩士 === 國立屏東科技大學 === 材料工程所 === 95 === This paper presents a micro flow cytometer with a 2-D hydrodynamic focusing and a micro-weir effect capable of concentrating particles/cells in the center of a sample stream for high-precision particles/cells counting. A simple and reliable isotropic wet etching fabrication process is used to fabricate the micro-weir structure in the cytometer on soda-lime glass substrates. The present micro flow system integrates three major modules, namely a hydrodynamically driven flow cytometer, a micro-weir structure in microchannel, and a laser induced fluorescence for particles/cells detection purposes. The results shown experimentally and numerically that particles/cells can be squeezed hydrodynamically into a narrow stream by two neighboring sheath flows and separated particles in vertical direction by micro-weir structure that they particles/cells individually through a detection region. The experimental and numerical results confirm the suitability of the proposed device for applications requiring the high-precision counting of particles or cells within a sample flow.