Stimulation of Poly I:C on TLR Gene Expressions in Porcine Antigen-Presenting Cells

• Main Authors: Chien-Wei Chen, 陳乾瑋
• Other Authors: Hso-Chi Chaung
• Format: Others
• Language: zh-TW
• Published: 2007
• Online Access: http://ndltd.ncl.edu.tw/handle/21686252055674103678

碩士 === 國立屏東科技大學 === 生物科技研究所 === 95 === Abstract Student ID: M9318004 Title of thesis: Stimulation of Poly I:C on TLR Gene Expressions in Porcine Antigen-Presenting Cells Total page: 66 Name of Institute: Graduate Institute of Biotechnology, National Pingtung University of Science ＆ Technology. Graduate date: 2007/07/12 Degree Conferred: Master Name of student: Chein-Wei Chen Adviser: Hso-Chi Chaung The contents of abstract in this thesis: Pattern recognition receptors (PRR) include two superfamilies, Toll-like receptors (TLR) and C-type lectin receptors. The expressions of PRRs on immune cells function to recognize different pathogen associated molecular patterns (PAMP) and thus induce different signal pathways in cell, which in turn causes the secretion of cytokines to regulate the acquired immune responses. Thirteen different TLRs have been demonstrated in human, however, there are only a few literatures on porcine TLRs. In this study, the effects of poly I:C on TLRs expressions of three porcine antigen-presenting cells including alveolar macrophage (AM), immature bone marrow haematopoietic-derived dendritic cells (BMHC-imDC), mature DC (BMHC-mDC) were investigated. The results showed that the TLR 3 and 8 gene expressions in AM were higher than those in imDC and mDC. No significantly higher expressions of TLRs were observed in mDC. Furthermore, the stimulation of poly I:C may inhibit the gene expressions of TLR 7 and 8 in AM and TLR8 in imDC but enhance the expression of TLR3 in AM. Under the stimulation of poly I:C, the expression of TLR9 in imDC was the highest among these three antigen-presenting cells.