Molecular phylogeny and identification on the different

• Main Authors: Pei-Yuan Lee, 李培源
• Other Authors: Chang-Hung Chou
• Format: Others
• Language: zh-TW
• Published: 2007
• Online Access: http://ndltd.ncl.edu.tw/handle/82777401356525748390

碩士 === 國立屏東科技大學 === 生物科技研究所 === 95 === Nucleotide variations of two rbcL - atpB and trnL - trnF noncoding spacer regions of the chloroplast DNA and microsatellite molecular markers were used to reconstruct molecular phylogenetics and to identity on the different varieties of momordica charantia L.. M. charantia, known as a species of Cucurbitaceae family, is a climbing annual vine and a popular vegetable in Asia. In this study, we used twenty-nine samples including seven wild populations, twenty cultivated varieties and two hybrid strains. The length of rbcL - atpB spacer in cpDNA was detected from 953 to 954 bps and only four mutation sites were found. The nucleotide diversity was 0.00108. All sample could separate to wild and cultivated group by using the demographic analysis. The length of trnL - trnF spacer in cpDNA was detected from 500 to 506 bps and eight mutation sites were found. The nucleotide diversity was 0.00644. One wild group and two cultivated groups, including white fruit and green fruit were shown. Two hybrids (H2 and H15) related to wild populations showing in NJ phylogenetic analysis by maternal inheritance data. In our result, single domestication events were proposed in bitter melon by genetic analysis. In order to understand the variations of nuclear genome, we use microsatellite markers to estimate the genetic variation and diversity by AMOVA and PCA analysis. In AMOVA test, the genetic variations among individuals are higher than among groups including total data, length data and mutation data sets. In PCA test, the wild individuals spread out in the demographic plot. The individuals of cultivated varieties were grouped into two areas in the demographic plot by the morphological character of the fruit color. Combined cpDNA and microsatellite results, we proprosed the single domestication events in bitter melon from wild to cultivated varieties.