Proteome analysis of potassium cyanide induced protein expression in Klebsiella oxytoca

• Main Authors: Chou Hui-hsuan, 周慧萱
• Other Authors: Chen Ssu-ching
• Format: Others
• Language: zh-TW
• Published: 2007
• Online Access: http://ndltd.ncl.edu.tw/handle/85536475820108120267

碩士 === 國立高雄師範大學 === 生物科技系 === 95 === Klebsiella oxytoca was isolated from the wastewater of a metal-plating plant that was shown to be able to biodegrade cyanide. In this study, we determined the ability of K. oxytoca to degrade cyanide (KCN) and the change of global proteins in this bacterium grown on KCN in relation to that grown on ammonia as the reference map using the analysis of 2D-electrophoresis coupled with MALDI-TOF MS. Results indicate that KCN could be degraded from 0.5 mM to 0.02 mM in 35 hours, and degraded from 1.0 mM to 0.01 mM in 55 hours, respectively. The protein profiling of K. oxytoca grown on KCN at middle log phase, late log phase and stationary phase, respectively, was determined. More than half (78%) of the identified proteins (>2-fold) were predicted to be involved in metabolic pathways of the bacterium. Proteins up-regulated during middle log phase include nitrogen limited induced protein and methane metabolism protein, but some stress protein like SOD was inhibited. Proteins up-regulated during late log phase include those involved in antioxidation and anabolic pathways. At stationary phase, TrpR protein was up-regulated controlled by stress gene rpoS, which was expressing at early stationary and stress condition. This study makes us to understand the physiological responses of K. oxytoca to the adaptation of the KCN which can aid to develop the mutant to degrade KCN efficiently.