Studies of Canavalia ensiformis lectin on the antitumor immunity in human leukemic U937 cells and the induction of proliferation and differentiation in human monocytes

• Main Authors: Shih-Hao Wang, 王識豪
• Other Authors: 江啟銘,廖慧芬
• Format: Others
• Language: zh-TW
• Published: 2007
• Online Access: http://ndltd.ncl.edu.tw/handle/84027883064591718509

碩士 === 國立高雄海洋科技大學 === 水產食品科學研究所 === 95 === The ancient traditional medicine has been reported that not only the beans of Canavalia ensiformis but also the pea pods can be used as therapeutic usage. For the harmful effects from the agglutinin activity, we tried to use the non-cytotoxic dosage with heating treatment to study the immunomodulatory effects on the human moncytes and the inhibitory effect on U 937 cell lines. It has shown that both the CEL and heating treated CEL (HCEL) exerted no cytotoxic effect on U937 at 30μg/mL. However, The MNC-CM( conditioned medium from monocyte cell) stimulated by both the CEL and HCEL at 20 μg/mL after 24 hours treatment can inhibit the growth of U937 and expression dose-dependent, respectively. The differentiate effect of CEL-MNC-CM can also observed as morphological change, from blast to matured or macrophages to about 7.5 ± 2.5 % in 20 μg/mL；NBT reduction up to 24.4 ± 3.2 % and expression dose-dependent. It is interesting that there is not any signification difference between HCEL and control group. The TNF-α、 IL-1β and IFN-γ can be found in CEL-MNC-CM, but only TNF-α and IL-1β in HCEL-MNC-CM. The obvious increment of human monocytes can also be observed after the stimulation by CEL and HCEL. We have confirmed that the proliferation of monocytes come from the immunomodulatory effects of CEL and HCEL on monocytes. Some kinds of cytokines or lymphokines were induced by monocytes to activate lymphocytes, substantially; the activated lymphocytes interact with monocytes to induce the proliferation of monocytes. In conclusion, the immunomodulatory effects of CEL and HCEL can induce the proliferation of monocytes and can induce the differentiation of U937.