| Summary: | 碩士 === 國立東華大學 === 生物技術研究所 === 95 === Diabetes mellitus (DM) is a complex metabolic syndrome with significant influence on the systemic and cerebral. The incidence and severity of ischemic stroke are deteriorated with the presence of high level plasma glucose, and outcome of stroke is inferior. In this study, high-fat fed rats were administrated with 30 mg/kg Bwt streptozotocin to induce type II diabetes (T2DM) and subsequently generated a focal ischemia model by using middle cerebral artery occlusion (MCAO) to mimic DM patients complicated with ischemic stroke. Results showed that Fat-Diabetes (Group FD) rats had the symptoms of high glucose level, insulin resistance, polyphagia, polydipsia and polyuria. Triphenyltetrazolium chloride staining showed that the cerebral infarct volume of Fat-Diabetes-Stroke (Group FDS) rats was larger than that observed in rats with Fat-Stroke (Group FS) and Normal-Stroke (Group NS) rats. Moreover, the extent of stroke was graded as level 3 after behavioral test. Additionally, expression levels of TNF-α and IL-1β mRNAs at 6 hours after transient MCAO in Group NS, Group FS and Group FDS rats were measured. TNF-α and IL-1β mRNAs expressed significantly in the cortex and hippocampus of injured hemisphere. Expression levels of TNF-α and IL-1β mRNAs in Group FDS were significantly higher than that of Group NS. The results also indicated that the changes in blood-brain-barrier (BBB) permeability of Group FDS were increased after 6 hours compared with Group FS and Group NS. According to the results, a rat model mimicing T2DM patients complicates with ischemic stroke has been successfully established. This model provides a new insight to propose that pro-inflammatory molecules such as TNF-α and IL-1β are critical causal factors for ischemic stroke damage followed by T2DM.
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