In vitro study of gallstone formation in simulated bile containing calcium ion

• Main Authors: Shih-Hung Chiu, 邱世鴻
• Other Authors: Chen-Lun Liu
• Format: Others
• Language: zh-TW
• Published: 2007
• Online Access: http://ndltd.ncl.edu.tw/handle/n4s8e2

碩士 === 國立東華大學 === 生物技術研究所 === 95 === Although patients with cholesterol gallstone rather then pigment gallstone were in the previously, the cholesterol gallstone cases are found quite often in the East now, because of a change of the hygienic condition, nutrition condition, and a westernization of the eating style. Patients of cholelithiasis are rising in such area of Hong Kong, Japan, Taiwan, and coastal of China. In addition, patients exhibit mixed gallstones of cholesterol and pigment are also common now in Taiwan. This may be due to a high content of calcium ion in drinking water. Because bile salts are anion biological surface-active agents, they may be act with calcium ions in bile. Therefore they have a buffered function of preventing calcium from precipitation. An increase of bile salt amount in bile will increase calcium solubility and suppress a formation of the pigment stones. Nevertheless previous studies have demonstrated that high bile salt / lecithin ratios in bile will cause high ChAT values, accelerates cholesterol crystallization and cholesterol gallstones. The present study investigated an interaction of calcium ions with two kinds of bile salts - Taurochenodeoxycholate (TCDC) and its epimer Tauroursodeoxycholate (TUDC). This result together with particle size analyzers measurements may provide more information of gallstone formation.