| Summary: | 碩士 === 國立嘉義大學 === 獸醫學系研究所 === 95 === Carbonic anhydrase (CA), a metallo-enzyme containing zinc, broadly distributes in mammalian tissues and participates in physiological regulation such as respiration, acid-base balance, ion transport, bone resorption, as well as the development of tumor by the reversible hydration of carbon dioxide. In this study we examine the histolocalization and gene expression of CA in both of normal canine mammary gland tissue and mammary gland tumor by histochemical, cytochemical, and RT-PCR. CA was located on the epithelium of acini, endothelium of blood vessels, cell membrane, vacuoles, space of acini, mitochondria, and erythrocyte of normal canine mammary glands. These findings may be related to the milking and the transport of CO2 and Ca2+ in the endothelium of mammary gland. Five CA isoenzymes mRNA expression, i.e. CA-II, CA-IV, CA-VI, CA-IX and CA-XII were found under RT-PCR analysis and showed different patterns between normal canine mammary gland tissue and canine mammary gland tumor tissue. High level of CA-VI and CA-IX gene mRNA expression and low level of CA-IV and CA-XII gene mRNA expression were found in the canine mammary gland tumor tissue. That high level of CA-VI and CA-IX gene mRNA expression might indicate that CA-VI and CA-IX are likely to be the essential enzymes to maintain the canine mammary gland tumor tissue cells living.
Key words: Carbonic anhydrase, mammary gland, localization, tumor, mRNA
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