| Summary: | 碩士 === 國立嘉義大學 === 食品科學系研究所 === 95 === Graptopetalum paraguayense belongs to Crassulaceae family, it has several health effects, such as lowering blood pressure, alleviating hepatic disorders and diuretic effects. Graptopetalum paraguayense applied in this research is from Alishan township, Chiayi county. The extract solvents are water, 50% ethanol, 70% ethanol and 95% ethanol. This study is aimed to evaluate the antioxidative and antimutagenicity activity, and to lead to the further investigation into the preventive effect of liver damage by using carbon tetrachloride and alcohol induced with Graptopetalum paraguayense.
According to antioxidative activity test, the result shows scavenging effect on DPPH free radical increases with the increase of Graptopetalum paraguayense concentration. Scavenging ability reaches above 80% whilst the extract’s concentration is 10 mg/mL. Scavenging effect on ABTS cation free radical of 50% ethanolic extract is the greatest. Another scavenging effect on nitric oxide of 70% ethanolic extract is the greatest. Reducing power of 95% ethanolic extract is the greatest whilst applying water extract is the lowest. Regarding scavenging effect of superoxide anion radical, water extract is the greatest. With regard to antioxidant constituent assay, the total phenolic and flavonoids contents of 95% ethanolic extract presents higher that are 32.5 mg/g and 17.39 mg/g. Cell cycle analysis reveals that the extracts of Graptopetalum paraguayense induce HepG2 cell via G1 cell cycle arrest. Furthermore, water extract among other ethanolic extracts has the greatest effect. In antimutagenicity assay, the result indicates the extracts from Graptopetalum paraguayense on the antimutagenicity of direct-acting mutagens is regards as better direct-acting mutagens.
In the plasm lipid study, this study is divided into Blank、Control、sample low dose group (50 mg/kg b.w./day)、medium dose (150 mg/kg b.w./day) and high dose (300 mg/kg b.w./day) five group. The data show that triglyceride, cholesterol and LDL-C levels were not significantly lowered in the Graptopetalum paraguayense water extracts. Alcohol-induced damage of tissue and GOT, GPT of blood is identified by feeding rats the Graptopetalum paraguayense water extracts. The results indicate that water extracts and silymarin significantly decreased the sGOT value at the high dosage group, and the effect is equal to those of silymarin. In conclusion, the water extracts from Graptopetalum paraguayense provide valuable information for inhibiter effect from CCl4 and the alcohol induced liver damage. Preventive effect of liver damage is found by using CCl4. Also, alcohol tends to induce by applying Graptopetalum paraguayense. The results show that by examining the damage caused by CCl4, the sGOT and sGPT are significantly different, and better than the blank group when feeding eight weeks. All samples (silymarin, low dose and high dose) significantly decrease the inhibition percent of sGOT 50, 70 and 75%. The inhibition percent of sGPT are 45 and 54% for low and high dose groups.
The research results provide valuable information for healthy food authorities and food industries.
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