| Summary: | 碩士 === 國立中央大學 === 機械工程研究所 === 95 === The progress of biotechnology not merely advents the human welfare, but reveals great economic potential. With the development of technology and the attraction of business profits, the biotechnology is destined to become a nouveau high-tech industry where the whole world will pour into enormous efforts.
This study aims to develop a container with liquid nitrogen at -196°C as a more suitable storage for slice of organism, by extracting advantage and eliminating limitation from currently-adopted containers. At present, there are the traditional manual lid-opening container by Taylor-Wharton, the mechanical container by Cryo-Cell (Cryo-Cell International Inc., USA), the one by BioArchive (Thermogenesis Corp., USA) and others in Taiwan.
The study procedure consists of two parts: container’s manufacture and software development. The former is achieved by the following steps: (1) draw the container model with 3D software; (2) calculate the structural strength with the finite-element analysis; (3) examine whether the stress concentration exceeds the material strength of the container; (4) manufacture the container and conduct the wear tests. The latter is to develop window-style software to control and monitor the operation and function of the container.
The results of the current study demonstrate the practicability of the new-designed container. Using this container, the operator could easily and conveniently put into and take off a single cartridge without any power supply, thus preventing the other cartridges in the same container from a decrease in the biological activity. Moreover, the development of the user-friendly software can facilitate and enhance the practicability and convenience of the hardware and software.
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