AFM Studies on Phospholipid Bilayers
碩士 === 國立中央大學 === 物理研究所 === 95 === We have studied the morphologies of DPPC, POPC, and POPC/DPPC membranes at room temperature using atomic force microscopy (AFM). The membranes were lipid bilayers supported on mica surfaces. Lipid bilayers prepared by spin coating and vesicle fusion were compared....
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| Format: | Others |
| Language: | en_US |
| Published: |
2007
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| Online Access: | http://ndltd.ncl.edu.tw/handle/90244912396137545872 |
| Summary: | 碩士 === 國立中央大學 === 物理研究所 === 95 === We have studied the morphologies of DPPC, POPC, and POPC/DPPC membranes at room temperature using atomic force microscopy (AFM). The membranes were lipid bilayers supported on mica surfaces. Lipid bilayers prepared by spin coating and vesicle fusion were compared. Both methods produce bilayers of similar thickness. However, spin coating results in more uniform lipid coverage. Because of the fluid nature of the bilayers, POPC (a low-melting lipid with Tm of -7 oC) present a more homogeneous morphology and a smaller bilayer thickness than DPPC (a high-melting lipid with Tm of 40 oC). The morphologies of binary mixtures of POPC and DPPC were obtained as a function of DPPC concentration. Lipid immiscibility is observed. Analysis of bilayer thickness suggests coexistence of POPC-rich and DPPC-rich domains. Addition of DPPC increases the fraction of the thicker-bilayer (i.e. DPPC-rich) domains, thus increase the average bilayer thickness of POPC/DPPC bilayers.
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