Development of a Multifunctional Optical Biosensor

• Main Authors: Wen-ying Chang, 張文穎
• Other Authors: Yuh-Shyong Yang
• Format: Others
• Language: en_US
• Published: 2007
• Online Access: http://ndltd.ncl.edu.tw/handle/98859085729845597981

碩士 === 國立交通大學 === 生化工程研究所 === 95 === This work presents a complementary metal oxide semiconductor (CMOS) chip with accompanied accessories as a system for the detections and quantifications of both biochemical luminescence and chromogens. The CMOS photosensing chip originality has been reported to detect multiple optical reactions, which detects not only chemiluminescence directly but also chromogen quantification while integrated with a commercial light emitting diode (LED), with a 1.28 mm× 1.28 mm detection area via a 0.35μm standard process. A transimpedance amplifier was designed in integrated circuit (IC) to amplify the signal current induced by luminescence or emitted light. By adjusting the frequency parameter setting of CMOS chip, the optimized scanning frequency can be set to fit different optical reactions. After the detected target is oxidized, hydrogen peroxide occurs. Horseradish peroxidase (HRP)–luminol–H2O2 and HRP-phenol-H2O2 systems were used as examples to constitute the platforms for coupling to produce different optical signals. Cholesterol–cholesterol oxidase (COD) and glucose-glucose oxidase(GOD) reactions were both coupled with different H2O2 production reactions to demonstrate the ability of biological luminescence and chromogen quantification and potential for diverse clinical diagnosis. Compared with the CMOS chip in the early stages, our chip has many advantages, such as high sensitivity, a less sample volume requirement, and high dynamic range. It even possesses higher unit sensitivity compared with the integrated commercial photodiode setup device. In conclusion, the combination of the specifically designed CMOS IC and available electronic devices establishes a useful and powerful multifunctional optical biosensor.