Immobilization and visualization of proteins on carbon nanotubes

• Main Authors: Ai-Huei Chiou, 邱薆蕙
• Other Authors: Chang-Ping Chou
• Format: Others
• Language: zh-TW
• Published: 2007
• Online Access: http://ndltd.ncl.edu.tw/handle/57966068793766077257

碩士 === 國立交通大學 === 機械工程系所 === 95 === In tradition methods, immobilization proteins on carbon nanotubes (CNTs), use acid treatment carbon nanotube(CNTs) to introduction carboxyl; Or utilize inertia metal, for example: Platinum, gold, silver. But acid treatment will be destroyed carbon nanotube, and utilize inertia metal, its price is more expensive and more difficult to make. So a thesis utilizes plasma treatment to modify CNTs surface to introduction amine groups (NH2). The base key is formed, use plasma treatment to introduce functional groups replace the traditional method and improve their shortcomings. Research to find out the optimum plasma treatment condition to immobilization a of proteins on carbon nanotubes (CNTs). Apply to biosensor (ex :FET-Biosensor..etc.) in the future. This thesis experiment is mainly divided into three parts: 1.CNTs synthesis Nickel catalytic-layers were deposited on silicon substrates by sputtering. Carbon nanotubes were prepared by the thermal CVD method. To demand CNTs growth under different conditions, and utilize the analytical instruments to observe changing of carbon nanotube. 2.Carbon nanotubes (CNTs) surfaces modify - Plasma treatment In this experiment, Plasma enhanced chemical vapor deposition (PECVD) system from carbon nanotubes (CNTs) by amino plasma treatment. Change of plasma treatment time, and utilize the analytical instruments to observe changing of carbon nanotube, The plasma treatment bind to amino groups incorporated into the CNTs surface. 3.Immobilization of proteins on CNTs Forced use glutaraldehyde(GA) cross-linking on the optimum plasma treatment condition of carbon nanotubes (CNTs).The experimental results show that a novel method of immobilizing mouse antibodies on CNTs was successfully developed by using amino plasma treatment.