Determination of Pentazocine in Urine by Gas Chromatography and Mass Spectrometry: Collaborative Study

• Main Author: 曲維蘭
• Other Authors: M-C Hsu
• Format: Others
• Language: zh-TW
• Published: 2007
• Online Access: http://ndltd.ncl.edu.tw/handle/61895522510544194524

碩士 === 國立體育大學 === 運動科學研究所 === 95 === Determination of Pentazocine in Urine by Gas Chromatography and Mass Spectrometry: Collaborative Study Abstract A sensitive and specific gas chromatography-mass spectrometer (GC-MS) method using selective ion monitoring (SIM) has been developed for quantification of pentazocine in urine specimen. This method using solid-solid extraction (SPE) and N,O-bis (trimethylsily) trifluoroacetamide with 1% trimethylchlorosilane derivatization, followed by GC-MS analysis using dextromethorphan as the internal standard. The method was linear from 62.5~2000 ng/mL. The limit of quantification and limit of detection were 62.5 and 31.25 ng/mL, respectively. The intraday precisions range from 6 to 10 %。The interday preciosions range from 4~11 %。This method was further subjected to an interlaboratory study. Nine laboratories participated in a study to analyze 3 samples in duplicate. Estimates for the repeatability and reproducibility of the method, expressed as relative standard deviations of the results of the determination of pentazocine in urine, were 3.0~5.2 % and 6.8~10.3 %, respectively. The HORRAT values among those 3 samples in duplicate are range from1.1 to 1.5. Therefore method reproducibility as normally would be expected according to the guidelines of AOAC. This method has been used in actual cases and appears to be reliable.