| Summary: | 碩士 === 國立暨南國際大學 === 應用化學系 === 95 === Analytical magnetapheresis has become a useful technique for analyzing magnetically susceptible particles. Magnetic particles ( Fe3O4 ) were prepared by co-precipitating.
The surface modifications of Fe3O4 were studied , transmission electron microscopy ( TEM ) , photon correlation spectroscopy ( PCS ) , atomic force microscope ( AFM ) , x-ray diffraction ( XRD ) , superconducting quantum interference device (SQUID) magnetometer , fourier transform infrared instruments ( FT-IR ) , UV-VIS and thermogravimetric analysis (TGA) were used for analysis.
The sizes of magnetic nanoparticles were :
Fe3O4 = 40 ± 2 nm、100 ± 5 nm、200 ± 5 nm and 350 ± 11 nm , and Fe3O4-polymer= 80 ± 5 nm、200 ± 8 nm、420 ± 10 nm and 860 ± 12 nm。
Anti-BSA or anti-CRP was bound onto Fe3O4-polymer particles. BSA or CRP was covalently bound PGMA particles, and applied to magnetic carriers for bioseparation in analytical magnetapheresis. We studied experimental parameters of magnetic particles、flow rate and particles number for analytical magnetapheresis.
From experimental result, the best conditions were 0.001ml/min of flow rate, and 125 particles 1000nm PGMA.
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