Development a PC12 Cell Cultivation and Monitoring System

• Main Authors: Chun-Jen Chien, 簡俊仁
• Other Authors: Kuo-sheng Cheng
• Format: Others
• Language: en_US
• Published: 2007
• Online Access: http://ndltd.ncl.edu.tw/handle/87438137097786698207

碩士 === 國立成功大學 === 醫學工程研究所碩博士班 === 95 === Cell line can be applied to provide the cell products, genetics, cell-cell interaction, environmental interaction, intracellular flux, and intracellular activity studies. The PC12 cell line is widely used in vitro study for investigating the neuronal apoptosis, neurochemistry, and neuronal differentiation. Therefore, the development of an appropriate cell cultivation platform as well as the real-time image acquisition and analysis system becomes an important issue. The purpose of this study is to develop a low-cost incubation system including microscopic image analysis system. The proposed system can acquire cell image for analysis during cell cultivation. From the experimental results, the temperature of incubation system ranges from 37.3 ± 1.3℃, and the lower bound of the relative humidity ranges from 89.7 ± 1.8%. The CO2 concentration is controlled at 5.29 ± 0.71%. The cell number is increased from 1.00 x 105 to 4.25 x 105 cells/ml after 48 hours cultivation. The cell viability test shows that the self-developed incubation system works well for PC12 cells cultivation; it can also acquire and segment the cell image during the cell cultivation. Besides, the region growing and modified blob labeling techniques are also employed to segment the PC12 cell image under cultivation conditions for later the morphology and feature quantification analysis.